anti fade mounting medium (MedChemExpress)
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Anti Fade Mounting Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+fade+mounting+medium/AntiFade+Mounting+Medium/pm42321182-423-15-18
Average 95 stars, based on 36 article reviews
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Incubation:Article Title: Surrogate production of ricefield eel sperm by germ cell transplantation in adult zig-zag eel Article Snippet: Sections on slides were blocked using 5% normal bovine serum (#A2153, Sigma Life Science, St. Louis, USA) in PBS +0.1% Triton-X100 (#V900502, VETEC, Sigma-Aldrich,Darmstadt, Germany) for 2 h. After wash, the primary Vasa antibody (GTX128306, GeneTex, Texas, USA) was added at dilution of 1:1000, and incubated overnight at 4 ◦C. .. Slides were washed 3 times with PBS for 5 min, followed by incubation in Alexa 488 or Alexa 555 (#A11008/A21428, Thermofisher, Waltham, USA) secondary antibodies (1:500) for 2 h. The samples were counter-stained with DAPI (#D9542, Sigma-Aldrich, 1:1000, Darmstadt, Germany) in 1× PBS at room temperature for 1 h. After three washing with PBS, slides were mountedusing an Fluorescence:Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways Article Snippet: TUNEL assay solution (C1086, Beyotime) was then added dropwise and incubated for 1.5 h. Subsequently, the samples were stained with DAPI solution (D9542, Sigma-Aldrich) for 10 min in darkness. .. After sealing with an Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury Article Snippet: The cells were incubated with DAPI (1 μ g/ml, cat. no. D669617, Shanghai Macklin Biochemical Co., Ltd.) at 25°C for 5 min in the dark to stain cell nuclei, followed by three additional rinses with PBS. .. Once sealed with Microscopy:Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways Article Snippet: TUNEL assay solution (C1086, Beyotime) was then added dropwise and incubated for 1.5 h. Subsequently, the samples were stained with DAPI solution (D9542, Sigma-Aldrich) for 10 min in darkness. .. After sealing with an Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury Article Snippet: The cells were incubated with DAPI (1 μ g/ml, cat. no. D669617, Shanghai Macklin Biochemical Co., Ltd.) at 25°C for 5 min in the dark to stain cell nuclei, followed by three additional rinses with PBS. .. Once sealed with Imaging:Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways Article Snippet: TUNEL assay solution (C1086, Beyotime) was then added dropwise and incubated for 1.5 h. Subsequently, the samples were stained with DAPI solution (D9542, Sigma-Aldrich) for 10 min in darkness. .. After sealing with an Software:Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury Article Snippet: The cells were incubated with DAPI (1 μ g/ml, cat. no. D669617, Shanghai Macklin Biochemical Co., Ltd.) at 25°C for 5 min in the dark to stain cell nuclei, followed by three additional rinses with PBS. .. Once sealed with Staining:Article Title: Frontotemporal dementia associated CHCHD10 V57E mutation aggravates tau pathology via disrupting the CHCHD10-Rab7A-TBC1D15 complex. Article Snippet: Post-coating, eye morphology was examined using a Teneo VS scanning electron microscope (FEI, USA) Immunofluorescence assay for Drosophila fat body tissues AR TI CL E IN P RE SS For Drosophila fat body analysis, dissected fat body tissues from 15 flies were fixed in 4% PFA at room temperature for 45 min, then washed three times with PBX (0.1% Triton X-100 in PBS). .. Tissues underwent direct DAPI staining before mounting onto objective slides (Sail Brand, 7101) using an Laser-Scanning Microscopy:Article Title: NSUN2 promotes colorectal cancer progression by stabilizing PHGDH mRNA to promote serine metabolism reprogramming Article Snippet: .. Finally, after three washes, the cells were mounted with |
