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anti fade mounting medium  (MedChemExpress)


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    Structured Review

    MedChemExpress anti fade mounting medium
    Anti Fade Mounting Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fade+mounting+medium/AntiFade+Mounting+Medium/pm42321182-423-15-18
    Average 95 stars, based on 36 article reviews
    anti fade mounting medium - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Surrogate production of ricefield eel sperm by germ cell transplantation in adult zig-zag eel
    Article Snippet: Sections on slides were blocked using 5% normal bovine serum (#A2153, Sigma Life Science, St. Louis, USA) in PBS +0.1% Triton-X100 (#V900502, VETEC, Sigma-Aldrich,Darmstadt, Germany) for 2 h. After wash, the primary Vasa antibody (GTX128306, GeneTex, Texas, USA) was added at dilution of 1:1000, and incubated overnight at 4 ◦C. .. Slides were washed 3 times with PBS for 5 min, followed by incubation in Alexa 488 or Alexa 555 (#A11008/A21428, Thermofisher, Waltham, USA) secondary antibodies (1:500) for 2 h. The samples were counter-stained with DAPI (#D9542, Sigma-Aldrich, 1:1000, Darmstadt, Germany) in 1× PBS at room temperature for 1 h. After three washing with PBS, slides were mountedusing an anti-fade mounting medium (#HY-K1042, MedChemExpress, USA). .. Mounted slides were imaged with a Leica Confocal Microscope (TCS SP8 STED, Wetzlar, Germany).

    Fluorescence:

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways
    Article Snippet: TUNEL assay solution (C1086, Beyotime) was then added dropwise and incubated for 1.5 h. Subsequently, the samples were stained with DAPI solution (D9542, Sigma-Aldrich) for 10 min in darkness. .. After sealing with an anti-fade mounting medium (HY-K1042, MedChemExpress), an inverted fluorescence microscope was used for observation and imaging. .. Lung tissues were collected and fixed in 2.5% glutaraldehyde (G6257, Sigma-Aldrich) at 4 ∘ C overnight.

    Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury
    Article Snippet: The cells were incubated with DAPI (1 μ g/ml, cat. no. D669617, Shanghai Macklin Biochemical Co., Ltd.) at 25°C for 5 min in the dark to stain cell nuclei, followed by three additional rinses with PBS. .. Once sealed with Anti-fade Mounting Medium (cat. no. HY-K1047, MedChemExpress), samples were observed using a fluorescence microscope, and 5 non-overlapping random fields of view were captured per sample to assess the apoptosis rate using the ImageJ 1.54 h software. .. BV2 cells were mixed with glutaraldehyde solution (2.5%, cat. no. G6257, Sigma-Aldrich; Merck KGaA) and fixed in the dark at 4°C for 4 h. After being washed twice with sterile PBS to remove glutaraldehyde, the samples were dehydrated with ethanol solution (30, 50, 70, 80, 90%) for 10 min each, then dehydrated with anhydrous ethanol.

    Microscopy:

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways
    Article Snippet: TUNEL assay solution (C1086, Beyotime) was then added dropwise and incubated for 1.5 h. Subsequently, the samples were stained with DAPI solution (D9542, Sigma-Aldrich) for 10 min in darkness. .. After sealing with an anti-fade mounting medium (HY-K1042, MedChemExpress), an inverted fluorescence microscope was used for observation and imaging. .. Lung tissues were collected and fixed in 2.5% glutaraldehyde (G6257, Sigma-Aldrich) at 4 ∘ C overnight.

    Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury
    Article Snippet: The cells were incubated with DAPI (1 μ g/ml, cat. no. D669617, Shanghai Macklin Biochemical Co., Ltd.) at 25°C for 5 min in the dark to stain cell nuclei, followed by three additional rinses with PBS. .. Once sealed with Anti-fade Mounting Medium (cat. no. HY-K1047, MedChemExpress), samples were observed using a fluorescence microscope, and 5 non-overlapping random fields of view were captured per sample to assess the apoptosis rate using the ImageJ 1.54 h software. .. BV2 cells were mixed with glutaraldehyde solution (2.5%, cat. no. G6257, Sigma-Aldrich; Merck KGaA) and fixed in the dark at 4°C for 4 h. After being washed twice with sterile PBS to remove glutaraldehyde, the samples were dehydrated with ethanol solution (30, 50, 70, 80, 90%) for 10 min each, then dehydrated with anhydrous ethanol.

    Imaging:

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways
    Article Snippet: TUNEL assay solution (C1086, Beyotime) was then added dropwise and incubated for 1.5 h. Subsequently, the samples were stained with DAPI solution (D9542, Sigma-Aldrich) for 10 min in darkness. .. After sealing with an anti-fade mounting medium (HY-K1042, MedChemExpress), an inverted fluorescence microscope was used for observation and imaging. .. Lung tissues were collected and fixed in 2.5% glutaraldehyde (G6257, Sigma-Aldrich) at 4 ∘ C overnight.

    Software:

    Article Title: Propofol upregulates MFG-E8 in BV2 cells to inhibit pyroptosis mediated by the NF-κB/NLRP3 pathway, thereby ameliorating ischemic-reperfusion neuronal injury
    Article Snippet: The cells were incubated with DAPI (1 μ g/ml, cat. no. D669617, Shanghai Macklin Biochemical Co., Ltd.) at 25°C for 5 min in the dark to stain cell nuclei, followed by three additional rinses with PBS. .. Once sealed with Anti-fade Mounting Medium (cat. no. HY-K1047, MedChemExpress), samples were observed using a fluorescence microscope, and 5 non-overlapping random fields of view were captured per sample to assess the apoptosis rate using the ImageJ 1.54 h software. .. BV2 cells were mixed with glutaraldehyde solution (2.5%, cat. no. G6257, Sigma-Aldrich; Merck KGaA) and fixed in the dark at 4°C for 4 h. After being washed twice with sterile PBS to remove glutaraldehyde, the samples were dehydrated with ethanol solution (30, 50, 70, 80, 90%) for 10 min each, then dehydrated with anhydrous ethanol.

    Staining:

    Article Title: Frontotemporal dementia associated CHCHD10 V57E mutation aggravates tau pathology via disrupting the CHCHD10-Rab7A-TBC1D15 complex.
    Article Snippet: Post-coating, eye morphology was examined using a Teneo VS scanning electron microscope (FEI, USA) Immunofluorescence assay for Drosophila fat body tissues AR TI CL E IN P RE SS For Drosophila fat body analysis, dissected fat body tissues from 15 flies were fixed in 4% PFA at room temperature for 45 min, then washed three times with PBX (0.1% Triton X-100 in PBS). .. Tissues underwent direct DAPI staining before mounting onto objective slides (Sail Brand, 7101) using an anti-fade mounting medium (MCE, HY-K1042) and square coverslips (Merck, BR470050). .. Images were acquired with a Zeiss LSM710 confocal microscope (Germany) equipped with a Plan-Apochromat 63x/1.40 Oil DIC M27 objective lens.

    Laser-Scanning Microscopy:

    Article Title: NSUN2 promotes colorectal cancer progression by stabilizing PHGDH mRNA to promote serine metabolism reprogramming
    Article Snippet: .. Finally, after three washes, the cells were mounted with Anti-Fade Mounting Medium (#HY-K1042, MedChemExpress, USA) and imaged using the LSM 900 Airyscan confocal laser-scanning microscope (Zeiss, Germany). ..



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    MedChemExpress dapi anti fade mounting medium
    A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with <t>DAPI-labeled</t> nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.
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    Image Search Results


    A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with DAPI-labeled nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.

    Journal: NPJ Vaccines

    Article Title: Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity

    doi: 10.1038/s41541-026-01458-0

    Figure Lengend Snippet: A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with DAPI-labeled nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.

    Article Snippet: Raw264.7, DC2.4, and Caco-2 cells in 24-well plates were incubated with RB-Ag-GPs for 4 h, fixed with 4% paraformaldehyde, stained with PKH67 green fluorescent cell linker kit (PKH67GL, Sigma-Aldrich, Germany) for membrane labeling and DAPI anti-fade mounting medium (DAPI, HY-K1047, MedChemExpress, USA) for nuclear staining, followed visualized by CLSM.

    Techniques: Staining, Labeling, Incubation, Ex Vivo, Fluorescence, Imaging, Flow Cytometry, Activation Assay, Single Cell, Selection

    A CLSM images of RB-Ag-GPs (red) uptake in Raw264.7 and DC2.4 cells pre-treated with PBS or LA (10 μg/mL, 2 h). Nuclei counterstained with DAPI (blue). B Ex vivo fluorescence imaging of FITC-Ag-GPs distribution in LA or PBS-pretreated mice at 4 h post-administration. Tissues analyzed: ILN, PP, MLN, IE, and major organs. C Dectin-1 mRNA expression levels in intestinal tissues of immunized mice at 7 dpi. Data are presented as mean ± SD ( n = 3). ns not significant, *** P < 0.01, **** P < 0.0001. D , E Larvae per gram (LPG) of muscle burden and the reduction rate in mice following T. spiralis infection with vaccination at 35 dpi. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01. F – H Ag-specific serum antibody levels (IgG, IgE, SIgA) in orally immunized mice. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Journal: NPJ Vaccines

    Article Title: Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity

    doi: 10.1038/s41541-026-01458-0

    Figure Lengend Snippet: A CLSM images of RB-Ag-GPs (red) uptake in Raw264.7 and DC2.4 cells pre-treated with PBS or LA (10 μg/mL, 2 h). Nuclei counterstained with DAPI (blue). B Ex vivo fluorescence imaging of FITC-Ag-GPs distribution in LA or PBS-pretreated mice at 4 h post-administration. Tissues analyzed: ILN, PP, MLN, IE, and major organs. C Dectin-1 mRNA expression levels in intestinal tissues of immunized mice at 7 dpi. Data are presented as mean ± SD ( n = 3). ns not significant, *** P < 0.01, **** P < 0.0001. D , E Larvae per gram (LPG) of muscle burden and the reduction rate in mice following T. spiralis infection with vaccination at 35 dpi. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01. F – H Ag-specific serum antibody levels (IgG, IgE, SIgA) in orally immunized mice. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Article Snippet: Raw264.7, DC2.4, and Caco-2 cells in 24-well plates were incubated with RB-Ag-GPs for 4 h, fixed with 4% paraformaldehyde, stained with PKH67 green fluorescent cell linker kit (PKH67GL, Sigma-Aldrich, Germany) for membrane labeling and DAPI anti-fade mounting medium (DAPI, HY-K1047, MedChemExpress, USA) for nuclear staining, followed visualized by CLSM.

    Techniques: Ex Vivo, Fluorescence, Imaging, Expressing, Infection